Meeting Archive:
3D Culture Methods and Imaging Considerations

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Meeting Description:
The use of three dimensional (3D) culture models is rapidly expanding due to their recognition as representing more structurally and physiologically relevant models of in vivo biology. 3D cell culture assays attempt to mimic the cellular microenvironment including in vivo extracellular matrix (ECM) composition. In this webinar we will cover the advantages and limitations of the various culture models, assay formats, and imaging approaches. Attendees will gain knowledge on setting up and optimizing a broad range of 3D assays that are the best fit for their particular application. Topics covered: 3D culture models, scaffold free vs. hydrogel matrix models, overview of the generation and use of organoids, molecular, biochemical and imaging assay methods.
Date: Wed, Apr 29, 2020
Time: 12:00 PM EDT
Duration: 1 hour
Host(s): BioTek
 Presenter Information
Brad Larson, Field Applications Scientist
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Brad joined BioTek in 2009 as a Principal Scientist in the Applications Group and now serves as a Field Scientist in the South Region. Brad’s early roles included optimizing new assay processes on BioTek’s line of automation, liquid handling, microplate detection, and imaging instrumentation; in addition to supporting new product development. Currently his role is supporting academic, biotech, and pharmaceutical researchers across five states. 


During his 25 year career in the Biotech Industry he has worked with numerous automation and detection platforms, as well as a variety of cell models, to optimize 2D and 3D cell culture assays across multiple research fields. His current work has led to publications in Assay and Drug Development Technologies, The Journal of Laboratory Automation, and  The Journal of Biomolecular Screening, among others.  Brad has additionally presented his work at numerous conferences and workshops across the United States, Europe, and Asia.    


Valerie Sodi, Field Applications Scientist
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Valerie Sodi earned her Ph.D. in Molecular and Cellular Biology and Genetics from Drexel University College of Medicine in Philadelphia and completed a Postdoctoral Fellowship at Fox Chase Cancer Center, where she studied mechanisms of ovarian cancer carcinogenesis and drug sensitivity/resistance.  She has both academic and industry experience in assay development, high throughput screening, and a variety of molecular biology and microscopy techniques, as well as a strong background in teaching.  She utilized 3D models of cancer in her graduate and postdoctoral work.