Meeting Archive:
Maximizing Fluorescent Signal Using Variable Bandwidth Monochromators for Plate Reader-Based Assay Optimization

This webinar has concluded. You can view the event recording here.
 
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Meeting Description:
One of the challenges with multiplexing fluorophores in biological assays is being able to reliably distinguish the signal of one fluorophore from another. The complication of working with fluorophores with significant spectral overlap is exacerbated when using fluorescent filters or fixed bandwidth monochromators, which provide little flexibility in optimizing signal and minimizing background. Variable bandwidth monochromators provide a powerful alternative that enables fine control over the light range by which fluorophores are excited and emitted light is collected, which in turn allows signal to be more reliably deciphered. BioTek offers a fleet of hybrid-plate readers including the Synergy H1, Neo2, and Cytation, which offer the most flexibility when assaying with multiple fluorescent probes. 
 
In this webinar, I will discuss the challenges of multiplexing fluorophores, and how variable bandwidth monochromators can maximize signal while minimizing background. In addition to providing general best practice guidelines for assay optimization, I will discuss how linear unmixing can be supplemented to decipher and refine signal coming from multiple fluorophores. 
 
Details
Date: Wed, Oct 28, 2020
Time: 12:00 PM EDT
Duration: 1 hour
Host(s): BioTek
 Presenter Information
Ernest Heimsath, Ph.D
Speaker Photo

Ernest Heimsath is a Principal Scientist in the Applications Group at BioTek Instruments, Inc. He earned his B.S. in Biology from UT San Antonio, and his Ph.D. in Biochemistry from Geisel School of Medicine at Dartmouth, where he studied the biochemical regulation of actin polymerization. Ernest conducted postdoctoral research at the National Institutes of Health and UNC School of Medicine, where he used advanced imaging techniques and animal models to define the contribution of actin-associated genes in building actin-based cellular structures crucial for cancer metastasis and organismal development. With expertise in fluorescence microscopy, his primary role at BioTek is to develop and optimize imaging-based applications.