Meeting Archive:
Using Automated Imaging to Quantitatively Analyze the Colony Formation Assay

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Meeting Description:
The colony formation assay evaluates the proliferative capacity of a single cell. For applications such as cancer drug screening, it is important to distinguish cells that retain this proliferative capacity from those that do not. In this webinar, we discuss automated methods for conducting and analyzing the colony formation assay using both the colorimetric and fluorescent properties of crystal violet, the well-established dye for this assay. We will cover important considerations for setting up the colony formation assay in multi-well formats, as well as discuss approaches for constructing a quantitative image analysis workflow that reliably identify colonies and extract meaningful results.
Details
Date: Wed, May 27, 2020
Time: 12:00 PM EDT
Duration: 1 hour
Host(s): BioTek
 Presenter Information
Ernest Heimsath, Ph.D.
Speaker Photo

Dr. Heimsath is a Principal Scientist in the Applications Group at BioTek — a part of Agilent. He earned his B.S. in Biology from UT San Antonio, and his Ph.D. in Biochemistry from Geisel School of Medicine at Dartmouth, where he studied the biochemical regulation of actin polymerization. Ernest conducted postdoctoral research at the National Institutes of Health and UNC School of Medicine, where he used advanced imaging techniques and animal models to define the contribution of actin-associated genes in building actin-based cellular structures crucial for cancer metastasis and organismal development. With expertise in fluorescence microscopy, his primary role at BioTek is to develop and optimize imaging-based applications.