In this webinar we will describe workflows and tips for optimizing a range of assays utilizing BioTek’s NEW Cytation 7 Cell Imaging Multi-Mode Reader. The Cytation 7 and its integrated upright microscope support an expanded range of applications including ELISpot, slide scanning with ROI identification, and colony formation assays.
The ELISpot assay is a powerful method for monitoring cellular immune responses to antigenic stimuli. Here we will describe variations on the ELISpot assay, including one- and two-color PVDP membrane-based formats, as well as methods utilizing silver staining. An integrated liquid handling instrument automates the most tedious and labor intensive aspect of this workflow, while automated image capture and analysis provides accurate spot counts based on sensitive size and intensity thresholds.
Slide scanning with advanced ROI identification: Cytation 7 scans samples at low magnification before prompting the user to identify regions of interest to be imaged at high magnification. This greatly accelerates the process of imaging ROIs in batches of complex microscopic samples. Examples of this feature set and relevant applications will be described.
Colony formation assay evaluates the proliferative capacity of a single cell. For applications such as cancer drug screening, it is important to distinguish cells that retain this proliferative capacity from those that do not. Conventional analysis of this assay involves scoring and quantifying colonies in each well of a multi-well format manually by eye, limiting its throughput capabilities. Here we will present an automated imaging-based method for conducting colony formation assays that delivers accurate and reproducible quantitative results.
